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Supported Tool

BUSCO assesses genome assembly and annotation completeness with Benchmarking Universal Single-Copy Orthologs.


BUSCO v2 provides quantitative measures for the assessment of genome assembly, gene set, and transcriptome completeness, based on evolutionarily-informed expectations of gene content from near-universal single-copy orthologs selected from OrthoDB v9.

The MultiQC module parses the short_summary_[samplename].txt files and plots the proportion of BUSCO types found. MultiQC has been tested with output from BUSCO v1.22 - v2.

File search patterns

  fn: short_summary*
  contents: "BUSCO version is:"
  num_lines: 1