Basic Statistics
Measure | Value |
---|---|
Filename | Sample-2.A005.C8LFMANXX.s_3.r_1.fq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 47405891 |
Sequences flagged as poor quality | 0 |
Sequence length | 125 |
%GC | 38 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC | 205927 | 0.4343911603728743 | TruSeq Adapter, Index 5 (100% over 50bp) |
AGCTTCTGAGAATGCTTCTGTCTAGAGTTTATATGAAGACAATCCCGTTT | 80543 | 0.1699008251949109 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 31235 | 0.0 | 88.41437 | 1 |
ATCGGAA | 33305 | 0.0 | 82.87437 | 2 |
TCGGAAG | 34740 | 0.0 | 79.516785 | 3 |
CGGAAGA | 35605 | 0.0 | 77.58375 | 4 |
TATGCCG | 27500 | 0.0 | 50.30099 | 46-47 |
TGCCGTC | 28085 | 0.0 | 48.903904 | 48-49 |
GCCGTCT | 28165 | 0.0 | 48.574883 | 48-49 |
CTCGTAT | 27490 | 0.0 | 48.33868 | 42-43 |
CGTATGC | 28815 | 0.0 | 48.108616 | 44-45 |
ATGCCGT | 28810 | 0.0 | 47.724674 | 46-47 |
TCTCGTA | 27105 | 0.0 | 47.59838 | 40-41 |
AGAGCAC | 59575 | 0.0 | 46.987 | 8 |
TCGTATG | 29680 | 0.0 | 46.62613 | 42-43 |
ATCTCGT | 28320 | 0.0 | 45.661327 | 40-41 |
GAGCACA | 62895 | 0.0 | 44.48781 | 9 |
CCGTCTT | 31240 | 0.0 | 43.91756 | 50-51 |
CGTCTGA | 31775 | 0.0 | 43.6548 | 16-17 |
CGTCTTC | 31445 | 0.0 | 43.565025 | 50-51 |
GATCTCG | 29440 | 0.0 | 43.489723 | 38-39 |
ACACGTC | 32055 | 0.0 | 43.4498 | 12-13 |