FastQCFastQC Report
Mon 18 Apr 2016
Sample-3.A006.C8H0KANXX.s_2.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSample-3.A006.C8H0KANXX.s_2.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70954559
Sequences flagged as poor quality0
Sequence length125
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC19857632.7986404650897767TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2520850.0102.569351
ATCGGAA2592100.099.759892
TCGGAAG2604700.099.218563
CGGAAGA2627450.098.301174
AGAGCAC3082550.083.8004158
GAGCACA3084300.083.704699
GAAGAGC3121350.082.8114556
AAGAGCA3536150.073.2393347
GGAAGAG3610850.071.759745
ATGCCGT2322950.056.03405846-47
GCCGTCT2324850.055.9266148-49
TCGTATG2340200.055.80587842-43
TCTCGTA2324050.055.76590740-41
CGCCAAT2241900.055.50512732-33
TATCTCG2274000.055.39738-39
TGCCGTC2322000.055.13881748-49
CGTATGC2341300.054.9205644-45
TATGCCG2338700.054.8421246-47
CTCGTAT2335450.054.8341442-43
CGTCTTC2371100.054.80298250-51